Christine Bole-Feysot

Available Equipments

The genomics core facility is equipped with:

• 1 sequencer NovaSeq6000, Illumina (production from 800 million to up to 10 billion of sequences/clusters)
• 1 sequencer iSeq100, Illumina (production of ~3 million of sequences/clusters)
• Access to a MiSeq, Illumina (production of up to ~15 million of sequences/clusters)

Other equipment:

• Capillary electrophoresis: Tape Station 2200 and 4200 (Agilent Technologies) Fragment Analyzer (Agilent Technologies) and Femto Pulse (Agilent Technologies)
• DNA fragmentation: Covaris E220
• Other devices for nucleic acid assay or quality control of NGS libraries:  Xpose spectrophotometer (Trinean), Qubit 4 fluorimeter (Invitrogen), Step One Plus Light Cycler (Life Technologies)

Services provided

The platform provides:

• Support for the experimental design
• Quality control of DNA, RNA and next-generation sequencing libraries
• Library Preparation and sequencing some types of next generation sequencing libraries
• Initial quality control of certain types of next generation sequencing libraries and their sequencing
• Initial quality control of the sequencing raw data and automatic transfer of the data to the bioinformatics platform.

Standard applications proposed (in collaboration with the bioinformatics platform for data analysis):

• Targeted re-sequencing: Exome sequencing and other types of targeted re-sequencing performed by hybridization capture
• mRNA-seq: 2 options for total RNA not degraded, one for total RNA quantities not limiting in the order of 100 ng to 1 µg, the other from 100 ng to 500 pg of total RNA)
• Deep-Amplicons-Seq: 2 technical options
• NovaSeq6000 FlowCell sequencing of various types of on-demand NGS libraries: Single Cell 10X Genomics libraries for example
• Other NGS sequencing applications: ChIP-Seq libraries sequencing (immunoprecipitated chromatin sequencing), ATAC-Seq, other amplicon sequencing, other types of applications on demand…